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目的 :观察抗纤合剂的抗肝纤维化作用。方法 :用日本血吸虫尾蚴感染新西兰兔 ,制成肝纤维化模型 ,病兔均以吡喹酮顿服杀虫治疗 ,继之中药组开始服用抗纤合剂治疗肝纤维化 ,并与秋水仙碱组及 0 .9%Na Cl组比较。RIA法检测血清 型前胶原 (PC )、透明质酸 (HA) ,苏木精 -伊红、天狼红染色观察胶原沉积 ,通过原位杂交检测基质金属蛋白酶 - 1(MMP- 1)和基质金属蛋白酶组织抑制剂 - 1(TIMP- 1)的表达 ,并作 、 型胶原和转化生长因子 β1 (TGF- β1 )免疫组化染色。结果 :中药组 PC 、HA水平降低 (P <0 .0 5 ) ,图像分析显示肝组织 、 型胶原和TGF- β1 表达减弱 (P <0 .0 5 ) ,肝组织 MMP- 1m RNA的表达增强 ,TIMP- 1m RNA表达减弱 (P <0 .0 5 ) ,肝纤维化程度减轻。结论 :抗纤合剂具有抗实验性血吸虫病兔肝纤维化的作用。
Objective: To observe the effect of anti-fibrosis agent on hepatic fibrosis. METHODS: New Zealand rabbits were infected with Schistosoma japonicum, and hepatic fibrosis model was established. All the diseased rabbits were treated with praziquantel, and then the Chinese medicine group began to take anti-fibrosis agent to treat hepatic fibrosis and colchicine group. And 0. 9% NaCl group comparison. ELISA was used to detect serotype procollagen (PC), hyaluronic acid (HA), hematoxylin-eosin staining, collagen deposition, and matrix metalloproteinase-1 (MMP-1) and matrix by in situ hybridization. Expression of tissue inhibitor of metalloproteinase-1 (TIMP-1), and collagen, and transforming growth factor-β1 (TGF-β1) immunohistochemical staining. Results: The levels of PC and HA in Chinese herbs group were decreased (P < 0.05). Image analysis showed that the expression of liver tissue, collagen and TGF-β1 was weakened (P <0.05), and the expression of MMP-1 mRNA in liver tissue was enhanced. TIMP-1 mRNA expression was decreased (P < 0.05), and the degree of hepatic fibrosis was reduced. Conclusion: Anti-fibrosis agent has anti-fibrosis effect in experimental schistosomiasis rabbits.