选用抗人Ｔ细胞ＣＤ３杂交瘤细胞产生的腹水，经系列免疫化学方法纯化和鉴定后，以异硫氰酸荧光素（ＦＩＴＣ）标记纯化之抗ＣＤ３单克隆抗体。将构建的荧光抗体与标准抗ＣＤ３单克隆抗体在流式细胞仪上分别对同一样本进行检测共２４例，ＣＤ３＋细胞分别是（６３８±４３）％和（６８２±４７）％，两者差异无显著性（Ｐ＞００５）。本文结果为进一步使国产抗ＣＤ系列单克隆抗体应用于流式细胞仪提供了基础 The ascites produced by anti-human T cells CD3 hybridoma cells were purified and identified by a series of immunochemical methods. The purified anti-CD3 monoclonal antibodies were labeled with fluorescein isothiocyanate (FITC). A total of 24 cases of CD3 + cells were detected by flow cytometry (638 ± 43%) and (682 ± 43), respectively, compared with the standard anti-CD3 monoclonal antibody 7)%, no significant difference between the two (P> 0  05). The results of this article will provide the basis for further application of domestic anti-CD monoclonal antibodies to flow cytometry