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聚焦层析法(focusing chromatography)是一种基于新的层析机理的快速纸上电层析法。其特点是在层析过程中,能使分离的物质聚焦(浓集)成线状谱(linear spectrum)层析带。近几年来,在放射性元素和核裂解产物等分离和鉴定上,已取得重要的应用。 1957年Schumacher首先利用这种技术分离了无机金属离子和放射性物质,并研究了它的基本理论。其后,品川睦明等更进一步阐明了层析机理及各种影响因素。从而使这一新技术在理论上和实践上都奠定了基础。在生化分析方面,Kolin等也发展了一种快速电层析法,并能获得聚焦层析的结果。但是其原理与本文所述者有所不同,须加以区别。聚焦层析法如与一般纸上电层析法相比较,具有
Fractionation chromatography is a fast paper electrokinetic chromatography based on a new chromatographic mechanism. It is characterized by the ability to focus (concentrate) the separated material into a linear spectrum in the chromatography process. In recent years, the separation and identification of radioactive elements and nuclear pyrolysis products have gained important applications. In 1957 Schumacher first used this technique to separate inorganic metal ions and radioactive material and to study its basic theory. Since then, Shinagawa Mu-ming and further clarify the chromatographic mechanism and a variety of influencing factors. So that this new technology has laid the foundation both in theory and in practice. In biochemical analysis, Kolin et al. Also developed a rapid electrokinetic method and obtained the results of Focused Chromatography. However, its principle is different from that described in this article, which needs to be distinguished. Focusing chromatography as compared with the general paper electrospray method, with