论文部分内容阅读
目的研究肝细胞生长因子及其信号通路激活与疟原虫肝细胞感染的关系。方法采用ELISA和Western blot来检测肝细胞生长因子。用FITC标记的葡聚糖吸收测定法检测肝细胞损伤,通过免疫荧光法观察肝细胞生长因子的表达与损伤的肝细胞的关系。同时,采用免疫共沉淀的方法检测肝细胞生长因子及其受体信号通路的激活以及磷酸化。结果疟原虫子孢子感染肝细胞后造成了肝细胞损伤,同时诱导了肝细胞生长因子的合成、表达和释放,肝细胞生长因子促使疟原虫子孢子感染肝细胞,肝细胞生长因子及其受体信号通路的激活是疟原虫子孢子感染肝细胞的必要前提。结论肝细胞生长因子及其受体可能是疟疾治疗的潜在靶点。
Objective To investigate the relationship between hepatocyte growth factor (HGF) and its signaling pathway activation and the infection of Plasmodium hepatocytes. Methods Hepatocyte growth factor was detected by ELISA and Western blot. The injury of hepatocytes was detected by FITC-labeled dextran absorption assay. The relationship between the expression of hepatocyte growth factor and the injured hepatocytes was observed by immunofluorescence. At the same time, immunoprecipitation method was used to detect the activation and phosphorylation of hepatocyte growth factor and its receptor signaling pathway. Results Plasmodium sporozoites induced hepatocellular injury after infection with hepatocytes and induced the synthesis, expression and release of hepatocyte growth factor. Hepatocyte growth factor (FH) induced Plasmodium sporozoites to infect hepatocytes, hepatocyte growth factor and its receptor Activation of the signaling pathway is a necessary prerequisite for infecting hepatocytes with Plasmodium sporozoites. Conclusion Hepatocyte growth factor and its receptor may be potential targets for the treatment of malaria.