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目的 探讨去松果体后其功能减退致褪黑素 (MT)分泌减少对大鼠学习能力及大脑皮质一氧化氮合酶 (NOS)表达的影响。方法 将大鼠进行 Y型迷宫测试 ,淘汰学习障碍的大鼠 ,将学习正常的大鼠随机分二组 ,实验组手术摘除松果体 ,对照组给予假手术 ,饲养 40天后再行 Y型迷宫测试 ,SABC法检测神经元型一氧化氮合酶 (n NOS)表达。结果 实验组大鼠学习能力明显低于对照组大鼠 (P<0 .0 1) ,而大脑皮质、内侧隔核 -斜角带核 (SM- DB) n NOS免疫反应阳性细胞增多 ,与对照组相比分别为 P<0 .0 5、P<0 .0 1,纹状体、海马与对照组相比 n NOS阳性细胞无明显差别 ,均 P>0 .0 5。结论 大鼠松果体摘除可以引起大鼠学习能力障碍 ,其原因可能与大脑皮质、SM- DB n NOS过度表达 ,引起 NO神经毒性有关。
Objective To explore the effect of decreasing the secretion of melatonin (MT) on the learning ability and the expression of nitric oxide synthase (NOS) in the cerebral cortex after dementia. Methods The rats were subjected to Y-maze test and the rats with learning disability were eliminated. The normal rats were divided into two groups at random. The pineal glands were removed in the experimental group and the sham operation was performed in the control group. After 40 days’ Test, SABC method to detect the expression of neuronal nitric oxide synthase (nNOS). Results The learning ability of rats in the experimental group was significantly lower than that in the control group (P <0.01), while the numbers of nSOS positive cells in the cerebral cortex and medial septum nucleus (SM-DB) increased. Compared with the control Compared with the control group, there was no significant difference in nNOS positive cells between the striatum and the hippocampus (P <0.05, P <0.01, P> 0.05). Conclusion Rat pineal gland ablation can cause learning disability in rats. The reason may be related to overexpression of SM-DBn NOS in the cerebral cortex and causing NO neurotoxicity.