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本文研究了从RSP-2·P3细胞系与正常人尿申分离纯化的两类集落刺激因子(RSP-2·P3-CSF与HU-CSF)的生物学特性。从集落生成的动态分析结果表明,这两类CSF的生物学活性是不均一的。在小鼠骨髓细胞的体外琼脂培养条件下,RSP-2·P3-CSF主要刺激粒系祖细胞(G-CFC)与粒-巨噬系祖细胞(GM-CFC)的增殖并生成由粒细胞(G-CFUc)或粒与巨噬细胞混合组成的集落(GM-CFUc);随着培养时间的延长,G-CFUc逐渐退化、消失,GM-CFUc则由最初主要为粒细胞组成逐渐过渡到粒与巨噬细胞的混合组成,最后生成由巨噬细胞组成的集落。然而,HU-CSF则主要刺激GM-CFC与巨噬系祖细胞(M-CFC)的增殖并生成相应的集落,随着体外培养时间的延长和HU-CSF浓度的增加,则巨噬细胞集落的比例急剧增高。
In this paper, the biological characteristics of two types of colony stimulating factor (RSP-2 · P3-CSF and HU-CSF) isolated and purified from RSP-2 · P3 cell line and normal urine were studied. Dynamic analysis of the generation of colonies from the colony indicates that the biological activity of these two types of CSF is heterogeneous. RSP-2 · P3-CSF mainly stimulates the proliferation of granulocyte-derived progenitor cells (G-CFCs) and granulocyte-macrophage-derived progenitor cells (GM-CFCs) under the condition of agar culture of mouse bone marrow cells in vitro, (G-CFUc) or granulocyte-macrophage colonies (GM-CFUc). With the prolongation of culture time, G-CFUc gradually degenerated and disappeared, and GM-CFUc gradually changed from the initial mainly granulocyte to Granules and macrophages mixed composition, the final formation of macrophages composed of colonies. However, HU-CSF mainly stimulated the proliferation of GM-CFC and macrophage-derived progenitor cells (M-CFCs) and generated corresponding colonies. As the in vitro culture time and HU-CSF concentration increased, macrophage colonies The proportion of sharp increase.