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【目的】水稻的抽穗期是决定水稻产量及其适用性的重要农艺性状之一,是由多基因控制的数量性状。染色体片段代换系减少了个体间遗传背景的干扰,已经成为定位和克隆复杂性状QTL的重要材料。【方法】本研究以9311为受体,日本晴为供体构建的128个重测序的染色体片段代换系群体为试验材料,利用多元回归,结合Bin-map图谱,【结果】鉴定到6个在南京、扬州不同年份间稳定表达的抽穗期QTL,其中,qHD2.1被定位在第2染色体上的759 848 bp区间内;qHD2.2被定位在第2染色体上的45 286 bp区间内;qHD 3.1被定位在第3染色体上的147 931 bp区间内;qHD5.1被定位在第5染色体上的213 351 bp区间内;qHD5.2被定位在第5染色体上的442 305 bp区间内;qHD8.1被定位在第8染色体上的538 176 bp区间内。【结论】本研究为精细定位并克隆相应QTL,进而探明抽穗期QTL的分子调控机制奠定了基础。
【Objective】 The heading date of rice is one of the important agronomic traits that determine the yield and its applicability of rice and is a quantitative trait controlled by polygenes. Chromosome segment substitution lines reduce the interference of genetic backgrounds among individuals and have become important materials for QTL mapping and cloning complex traits. 【Method】 In this study, 128 recombined chromosomal segment substitution lines with 9311 as acceptor and Nipponbare as donors were used as experimental materials. By using multiple regression and Bin-map, the results showed that 6 Nanjing, and Yangzhou. Among them, qHD2.1 was located in the region of 759 848 bp on chromosome 2; qHD2.2 was located in the region of 45 286 bp on chromosome 2; qHD 3.1 was mapped on the 147 931 bp interval on chromosome 3; qHD5.1 was mapped on the 213 351 bp interval on chromosome 5; qHD5.2 was mapped on the 442 305 bp interval on chromosome 5; qHD8 .1 is located within the 538 176 bp interval on chromosome 8. 【Conclusion】 This study lays the foundation for the fine mapping and cloning of QTLs and the identification of the molecular regulation mechanism of QTL at heading stage.