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1980年Zimmermann报告了电场诱导细胞融合的新技术。它主要包括两个电过程:一是以电介质电泳使细胞形成“串珠”,达到细胞紧密接触;二是以瞬间直流高压电脉冲使细胞发生可逆电穿孔,相邻细胞的细胞质沟通,达到细胞融合。由于这一技术具有可使差异较大的细胞发生融合,在显微镜下能观察融合过程,物理刺激对细胞无毒性,融合率较PEG法高等优点。引起了许多学者的兴趣。现已在植物细胞,动物细胞,菌细胞,以及脂质体、质粒与细胞的融合等方面进行了广泛的应用研究。我们用这一技术建立鼠B淋巴细胞杂
In 1980 Zimmermann reported a new technique of electric field-induced cell fusion. It mainly includes two electrical processes: one is the electrophoresis of the cells to form “beads” to achieve close cell contact; the second is the instant direct high voltage pulse to reversibly electroporate the cells, and the cytoplasm of neighboring cells communicates to reach the cell. Fusion. Because this technology allows the fusion of the larger cells, the fusion process can be observed under the microscope. The physical stimulation is not toxic to the cells, and the fusion rate is higher than the PEG method. Has attracted the interest of many scholars. Nowadays, extensive application research has been carried out in plant cells, animal cells, bacterial cells, liposomes, plasmids and cells. We used this technique to establish murine B lymphocyte heterogeneity