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一、液体菌种的生产:供试菌株CoV-1,由上海师大菌蕈研究室提供。液体菌种F_1、F_2及F_3按上海师大“食用菌深层培养”专利配方进行配制。将配制好的F_1培养基分装于250ml锥形瓶中,每瓶装量为50ml。灭菌压力1.1kg/cm~2,温度121℃,维持40分钟。灭菌后烘干棉塞,移入无菌室接种箱内,打开紫外灯,待培养液温度降至30℃即可进行按种。菌种应选用新鲜正在生长的斜面母种。F_1种子的培养采用振荡培养法,摇瓶机的转速为220转/分,培养室温度控制在28-30℃,经48小时的振荡培养即可转接F_2。将配制好的F_2培养基分装于500ml锥形瓶中,每瓶装量为100ml,灭菌压力1.1kg/cm~2,温度121℃,维持40分钟。灭菌后烘干棉塞随即移入无菌室接种箱内。F_1种子放入接种箱之前,用0.1％的新洁尔灭清洗种瓶外壁,洗净后放入接种箱内打
First, the production of liquid strains: test strains CoV-1, provided by the Shanghai Normal University fungus mushroom laboratory. Liquid strains F_1, F_2 and F_3 were formulated according to the patent formula of “deep culture of edible fungi” of Shanghai Normal University. Prepared F_1 medium sub-250ml conical flask, the amount of each bottle is 50ml. Sterilization pressure 1.1kg / cm ~ 2, temperature 121 ℃, maintained for 40 minutes. Sterilized and dried tampon, transferred to the sterile room inoculation box, turn on the UV lamp, until the temperature of the culture solution dropped to 30 ℃ can be carried out by species. Species should be selected fresh growing beveled mother species. F_1 seeds were cultured by shaking culture method. The speed of shake flask was 220 rpm and the temperature in the culture chamber was controlled at 28-30 ℃. The F_2 seeds could be transferred by shaking for 48 hours. The prepared F_2 medium was subdivided into 500ml Erlenmeyer flasks, the volume of each bottle was 100ml, the sterilization pressure was 1.1kg / cm ~ 2, the temperature was 121 ℃ and maintained for 40 minutes. Sterilized tampon then moved into the sterile room vaccination box. F_1 seeds into the inoculation box, with 0.1% benzalkonium bromide wash the outer wall of the bottle, washed into the inoculation box