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经气管内滴入猪胰弹性蛋白酶(PELT)复制金黄地鼠肺气肿模型。收集支气管肺胞灌洗液(BALF),观察其中弹性蛋白酶(ELT)、α_1-抗胰蛋白酶(α_1-AT),β-葡萄糖醛酸酶(β-g)活性等的变化,有助于阐明肺气肿的发病机理,并为防治提供实验资料。金黄地鼠,雄性,体重100～120g,经气管内滴入PELT(常州生化试剂厂提供),用量100U/100g体重;两个月后处死动物,检测肺动脉压,右心室/左心室+室间隔(R/L+S)比值,常规收集BALF,计数细胞;以弹性蛋白为底物,琼脂糖板扩散法测定ELT活性,以BAPNA为底物比色法测定α_1-AT活性;以PPGS为底
The model of emphysema in golden hamster was transplanted with porcine pancreatic elastase (PELT). The bronchoalveolar lavage fluid (BALF) was collected and observed for changes in the activity of elastase (α1-AT) and β-glucuronidase (β-g) Emphysema pathogenesis, and provide experimental data for prevention and treatment. Golden hamster, male, weight 100 ~ 120g, endotracheal instillation of PELT (Changzhou biochemical reagent factory), the amount of 100U / 100g body weight; two months after the animals were sacrificed to detect pulmonary artery pressure, right ventricular / left ventricular + (R / L + S) ratio, BALF was collected routinely, and the cells were counted. ELT activity was measured by agarose plate diffusion with elastin as substrate. The activity of α_1-AT was measured by BAPNA substrate assay.