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【目的】前期研究表明总氮浓度为5mmol/L,铵硝配比为10∶90可以促进弱光胁迫下娃娃菜幼苗的生长,本试验验证一氧化氮(NO)是否参与铵硝营养缓解弱光下娃娃菜幼苗生长及抗性的调节,以期为实际生产提供理论依据.【方法】以娃娃菜幼苗为试验材料,采用营养液栽培,处理第8d,对其抗氧化酶活性、丙二醛及脯氨酸含量进行测定.【结果】弱光下,单独NO供体硝普钠(SNP)处理后,抗氧化酶SOD活性显著高于铵硝比10∶90处理(CK),丙二醛(MDA)含量显著小于CK,说明SNP可缓解弱光胁迫对娃娃菜幼苗的氧化损伤;铵硝比中添加NO抑制剂(L-NAME,NaN3)和清除剂Hb后,地上地下鲜重及总叶面积较CK减小,SOD活性、MDA和Pro含量显著增加.【结论】NO抑制剂和清除剂逆转了铵硝对弱光胁迫下植株抗氧化系统及细胞膜的保护作用.因此,NO参与了铵硝比10∶90缓解弱光胁迫对娃娃菜幼苗的SOD活性、MDA及Pro含量的调节.
【OBJECTIVE】 Previous studies showed that total nitrogen concentration of 5 mmol / L and ammonium nitrate ratio of 10: 90 could promote the growth of D. chinensis seedlings under low light stress. This experiment verified whether nitric oxide (NO) participates in the alleviation of ammonium nitrate nutrition weakly In order to provide a theoretical basis for the actual production, the method was used to establish the theoretical basis for the actual production.METHODS With the saplings as the experimental material, the nutrient solution was used for cultivation for 8 days, and its antioxidant enzyme activities, malondialdehyde And proline content were measured. 【Result】 Under low light, SOD activity of antioxidant enzyme SOD was significantly higher than that of NO treatment 10: 90 (CK), malondialdehyde (MDA) was significantly lower than that of CK, indicating that SNP could alleviate the oxidative damage to seedlings of Brassica chinensis under low light stress. After addition of NO inhibitor (L-NAME, NaN3) and scavenger Hb, the above-ground fresh weight and total Leaf area decreased compared with CK, SOD activity, MDA and Pro content increased significantly. 【Conclusion】 NO inhibitors and scavengers reversed the protective effect of ammonium nitrate on antioxidant system and cell membrane of plants under low light stress. Ammonium ratio 10: 90 mitigates SOD activity, MDA and Pro in weak light stress on baby The amount of the adjustment.