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目的探讨十溴联苯醚(PBDE-209)暴露对小鼠学习记忆能力的影响和可能机制。方法将18只4周龄雄性ICR小鼠随机分成3组,每组6只。分为溶剂对照组(DMSO组),低剂量染毒组(300 mg/kg),高剂量染毒组(500 mg/kg),每周染毒6次,连续染毒6周。染毒第6周利用Morris水迷宫测定小鼠学习记忆能力(期间继续给药),染毒6周结束后处死老鼠,取一侧海马进行HE染色观察海马组织形态学改变,另一侧海马测定组织NOS活性和NO含量。结果小鼠海马组织的病理切片结果显示:染毒组海马组织CA1区细胞排列紊乱,高剂量染毒组的细胞紊乱更明显。Morris水迷宫实验结果显示,高剂量染毒组和低剂量染毒组小鼠找到平台的平均潜伏期分别为(64.322±4.419)s和(50.912±4.419)s,长于对照组的(23.183±4.419)s(P<0.05)。探索实验结果显示,高剂量染毒组穿越平台次数为(1.83±0.753)次,明显少于对照组的(3.67±0.516)次(P<0.05)。高剂量染毒组和低剂量染毒组小鼠海马NOS活性分别是(1.209±0.241)U/mg prot和(2.198±0.192)U/mg prot,低于对照组的(3.551±0.347)U/mg prot;NO含量分别为(0.099±0.012)μmol/mg prot和(0.325±0.027)μmol/mg prot,低于对照组的(0.544±0.020)μmol/mg prot(P<0.05)。结论 PBDE-209暴露可能抑制海马中NOS活性及NO含量,并影响小鼠学习记忆能力,其间关系值得进一步探讨。
Objective To investigate the effects and possible mechanisms of PBDE-209 exposure on learning and memory in mice. Methods Eighteen 4-week-old male ICR mice were randomly divided into 3 groups with 6 mice in each group. The rats were divided into DMSO group, low dose group (300 mg / kg) and high dose group (500 mg / kg). The animals were treated 6 times a week for 6 weeks. At the 6th week of exposure, the learning and memory abilities of mice were measured by Morris water maze, and the mice were sacrificed at the end of the 6th week. The hippocampus was harvested for HE staining to observe the change of hippocampal morphology. On the other side, Tissue NOS activity and NO content. Results The pathological sections of hippocampus of mice showed that the cells in hippocampus CA1 were disordered and the cells in high dose were more disordered. The results of Morris water maze test showed that the average incubation period of platform found in the high-dose and low-dose groups was (64.322 ± 4.419) s and (50.912 ± 4.419) s, respectively, which was longer than that of the control group (23.183 ± 4.419) s (P <0.05). The experimental results showed that the number of high-dose exposure groups across the platform was (1.83 ± 0.753) times, significantly less than that of the control group (3.67 ± 0.516) times (P <0.05). The NOS activity of hippocampus in hippocampus of high dose and low dose groups were (1.209 ± 0.241) U / mg prot and (2.198 ± 0.192) U / mg prot, respectively, which were lower than that of control group (3.551 ± 0.347) U / The contents of NO and NO were (0.099 ± 0.012) μmol / mg prot and (0.325 ± 0.027) μmol / mg prot, respectively, lower than that of the control group (0.544 ± 0.020) μmol / mg prot. Conclusion PBDE-209 exposure may inhibit NOS activity and NO content in hippocampus and affect learning and memory in mice, and the relationship between them deserves further exploration.