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采用焦锑酸钙沉淀的电镜细胞化学方法 ,研究了低温胁迫与冷锻炼下不同耐冷性番茄品种幼叶细胞Ca2 + 定位分布的变化。结果表明 :正常生长条件下 ,番茄幼叶细胞Ca2 +主要存在于液泡和细胞间隙内 ,细胞质中含量很低 ,且耐冷性不同品种之间无明显差异。经5℃低温处理 2 4h后 ,耐冷品种细胞内Ca2 + 分布变化明显 ,胞内钙库 (即液泡 )释放Ca2 + 进入细胞基质 ,细胞间隙中仍有大量Ca2 + 存在 ;而冷敏感品种无明显变化。当处理 4 8h后 ,耐冷品种细胞Ca2 + 分布趋向于恢复到处理前的状态 ,而冷敏感品种则在细胞内形成较大的钙沉淀颗粒 ,多分布于叶绿体被膜与质膜内侧 ,细胞间隙内Ca2 + 颗粒也集中聚集成团。昼 15℃ /夜 8℃条件下的冷锻炼对不同耐冷性番茄幼叶细胞内Ca2 + 分布的影响也表现不同
The changes of Ca2 + localization in young leaf cells of cold-tolerant tomato cultivars under chilling stress and cold acclimation were studied by electron microscopy cytochemistry with calcium pyroantimonate precipitation. The results showed that under normal conditions, Ca2 + in young leaves of tomato mainly existed in the vacuole and intercellular space, the contents of cytoplasm were very low, and there was no significant difference between different cold tolerance cultivars. The intracellular Ca2 + distribution of cold-tolerant cultivars changed obviously after low temperature treatment at 5 ℃ for 24 hours, and Ca2 + release from intracellular calcium pool (vacuole) into the matrix of cells, and there was still a large amount of Ca2 + in the intercellular space. Variety. When treated for 48 h, the distribution of Ca2 + in cold-tolerant cultivars tended to return to their pre-treatment state, whereas cold-sensitive cultivars formed larger precipitates of calcium in the cells, mostly in the chloroplast envelope and the plasma membrane, and in the intercellular space Ca2 + particles are also concentrated into clusters. The effects of cold acclimation at 15 ℃ / night at 8 ℃ on intracellular Ca2 + distribution in young leaves with different cold tolerance were also different