论文部分内容阅读
应用细胞增殖动力学和流式细胞分析术检测入白细胞干扰素对人鼻咽癌上皮样细胞株（ＣＮＥ）的作用。结果表明干扰素抑制ＣＮＥ细胞的增殖，并使ＣＮＥ细胞的ＤＮＡ含量由超三倍体分化成近三倍体类型。用干扰素４００ＩＵ／ｍｌ后，２ｄ内Ｇ２＋Ｍ和Ｇ１时相时间延长；干扰素１２００ＩＵ／ｍｌ用药后２～６ｄ内Ｇ１时相时间长于低剂量组，且用药后２～８ｄ内Ｓ时相时间缩短。因鼻咽癌是以放射治疗为主的疾病，而Ｓ时相细胞对放射抗拒，故干扰素在鼻咽癌的治疗中可能有一定的应用价值。
Cell proliferation kinetics and flow cytometry were used to detect the effect of IFN-γ on human nasopharyngeal epithelial cell line (CNE). The results indicate that interferon inhibits the proliferation of CNE cells and causes the DNA content of CNE cells to differentiate from the triploid to the nearly triploid type. After treated with 400IU / ml interferon, G2 + M and G1 phase prolonged within 2d; G1 phase time was longer than low dose group within 2 ~ 6d after treatment with 1200IU / ml, and S phase time was shortened within 2 ~ 8d after treatment . Due to nasopharyngeal carcinoma is a radiation-based disease, and S-phase cells on the radiation resistance, so interferon in the treatment of nasopharyngeal carcinoma may have some value.