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目的 :研究珍珠梅对二乙基亚硝胺致大鼠肝脏癌前病变灶及抗氧化活力的抑制作用。方法 :75只大鼠随机分为A、B、C 3组 :对照组、模型组和治疗组 ,每组 2 5只。按略改良的Solt Farber氏方法制作大鼠肝脏癌前病变模型 ,用珍珠梅提取物饲养大鼠 6周后处死 ,通过免疫组织化学检测癌前病变组织谷胱甘肽S -转移酶 (GST P)和p5 3蛋白的表达 ,放射免疫法检测血清肿瘤坏死因子 (TNF α)的含量 ,比色分析法检测血清、肝线粒体超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH Px)的活性及丙二醛(MDA)的含量。结果 :B和C组GST P的表达率为 86 7%和 2 8 6 % ,p5 3的表达率为 5 6 %和 36 % ,差异均有显著意义 ,P <0 0 5 ;B组血清TNF α的含量与C组比较 ,差异有显著意义 ,P <0 0 5 ;B组血清、肝线粒体SOD、GSH Px活性及MDA含量与C组比较 ,差异均有显著意义 ,P <0 0 5。结论 :珍珠梅提取物对DEN所致大鼠癌前病变灶有抑制作用 ,可诱发TNF α的生成 ,并有抗氧化作用。
Objective: To study the inhibitory effect of pearl plum blossom on precancerous lesion and antioxidant activity of rat induced by diethylnitrosamine. Methods: Seventy five rats were randomly divided into A, B and C groups: control group, model group and treatment group, 25 rats in each group. The rat liver precancerous lesion model was made according to the slightly modified Solt Farber’s method. The rats were sacrificed 6 weeks after the rats were pruned with the plum plum extract, and the expression of glutathione S-transferase (GST P ) And p5 3 protein were detected by enzyme-linked immunosorbent assay (ELISA). The level of tumor necrosis factor (TNF) was measured by radioimmunoassay. Serum and liver mitochondrial superoxide dismutase (SOD) and glutathione peroxidase GSH Px) activity and malondialdehyde (MDA) content. Results: The expression rates of GST P in group B and group C were 86.7% and 286%, while the expression rates of p5-3 were 56% and 36% respectively (P <0.05) (P <0.05). The activities of SOD, GSH Px in serum and liver mitochondria of group B and MDA content in group B were significantly different from those in group C (P <0.05). Conclusion: The extract of pearl plum can inhibit the precancerous lesion of rats induced by DEN, induce the production of TNFα, and have anti-oxidative effects.