脑组织(核团)推挽灌流法,是分析脑内化学物质含量变化时常用的收集样品的方法。其特点是能在清醒动物身上进行动态观察,因而在开展神经肽和中枢神经递质与针刺镇痛的关系等研究时得到较广泛的应用。这种方法不需要复杂的设备,但需要有一个合适的推挽灌流套管。常用的套管一般由不锈钢材料制成,优点是可以重复使用,但要求内芯与外套管座的接口严密不漏水,因而机械加工时有一定难度,而且成品批量小成本高,目前很少有厂家愿意生产。若用硬塑料材料制作,一则不能重复使用,二则同样也有一定难度.这就实际上限制了这种方法的进一步普及推广。本实验室在科研实验中 Brain tissue (nucleus) push-pull perfusion method is used to analyze changes in the content of chemical substances in the brain to collect samples. It is characterized by dynamic observation in conscious animals, and thus in the study of the relationship between neuropeptide and CNS neurotransmitter and acupuncture analgesia has been more widely used. This method does not require complicated equipment, but requires a suitable push-pull irrigation cannula. Commonly used casing is generally made of stainless steel, the advantage is that you can re-use, but the inner core and the outer sleeve requires strict interface tight watertight, so there is a certain degree of difficulty machining, and finished bulk small high cost, there are very few Manufacturers are willing to produce. If made of hard plastic material, one can not be reused, the second is also a certain degree of difficulty.This actually limits the further popularization of this method. The laboratory in scientific research experiments