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目的探讨基质金属蛋白酶-2、-9(MMP-2、MMP-9)及其组织抑制因子-1、-2(TIMP-1、TIMP-2)在不同分期子宫内膜异位症(EMs)患者血清及腹腔液中的表达。方法收集2011年1月~2013年12月确诊的70例EMs患者和30例对照组血清和腹腔液,用酶联免疫吸附法(ELISA)检测MMP-2、MMP-9、TIMP-1和TIMP-2水平,并进行比较分析。结果 MMP-2和MMP-9在EMs组血清及腹腔液中的表达显著高于对照组、Ⅲ-Ⅳ期显著高于Ⅰ-Ⅱ期、血清中的表达显著高于腹腔液(P<0.05)。TIMP-1和TIMP-2在对照组血清及腹腔液中的表达显著高于EMs组、Ⅰ-Ⅱ期显著高于Ⅲ-Ⅳ期、腹腔液中的表达显著高于血清(P<0.05)。结论 MMP-2和MMP-9的高表达、TIMP-1和TIMP-2的低表达在子宫内膜异位症的发病和发展过程中可能起重要的作用。
Objective To investigate the expressions of MMP-2, MMP-9, TIMP-1 and TIMP-2 in patients with different stages of endometriosis (EMs) Patient serum and peritoneal fluid expression. Methods Serum and peritoneal fluid were collected from 70 patients with EMs and 30 controls from January 2011 to December 2013. The expressions of MMP-2, MMP-9, TIMP-1 and TIMP were detected by enzyme linked immunosorbent assay (ELISA) -2 levels, and comparative analysis. Results The expression of MMP-2 and MMP-9 in serum and peritoneal fluid of EMs group was significantly higher than that of the control group (P <0.05), the expression of MMP-2 and MMP-9 in serum and peritoneal fluid was significantly higher than that in the control group . The expressions of TIMP-1 and TIMP-2 in the serum and peritoneal fluid of the control group were significantly higher than those in the EMs group. The expression of TIMP-1 and TIMP-2 in the peritoneal fluid was significantly higher than that in the serum (P <0.05). Conclusion The high expression of MMP-2 and MMP-9, the low expression of TIMP-1 and TIMP-2 may play an important role in the pathogenesis and progression of endometriosis.