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选取高低温分别胁迫、协同胁迫以及超声波处理等方式研究了短期物理应激对云芝菌丝体超氧化物歧化酶(SOD)活性的影响。与未作胁迫处理的对照相比,在低温处理中,10℃较之15℃处理SOD活性整体水平有明显提高,10℃处理2h可使SOD活性提高36.2%,延长处理时间导致SOD活性下降;高温协迫中30℃处理效果优于40℃、50℃和60℃,30℃处理4h,SOD相对酶活可提高至26.8%,延长处理时间和提高处理温度均导致SOD活性下降。高低温协同作用L16(2×42)正交试验结果分析表明,30℃处理2h后10℃处理1.5h,SOD相对酶活显著提高,达54.6%,但高低温处理的顺序对SOD活性影响无差异。超声波实验表明,超声波38kHz处理40min,SOD活性比对照提高41.9%,延长处理时间酶活显著下降。结果表明,高低温分别胁迫、协同胁迫以及超声波处理等短期物理应激在一定的应激强度和一定的处理时间范围内对云芝菌丝体SOD的活性有提高作用,其中高低温协同胁迫提升SOD活性的效果比单温处理更显著。
The effect of short-term physical stress on superoxide dismutase (SOD) activity of mycelia was studied by means of high and low temperature stress, coordinated stress and ultrasonic treatment. Compared with the unstressed control, the SOD activity at 10 °C was significantly higher than that at 15 °C in the low temperature treatment. SOD activity was increased by 36.2% at 10 °C for 2 h, and the SOD activity was decreased by prolonged treatment time. The effect of 30 °C treatment in high temperature coercion was better than that of 40 °C, 50 °C and 60 °C, 4 h treatment at 30 °C, SOD relative enzyme activity could be increased to 26.8%, prolonging the treatment time and increasing the treatment temperature all resulted in the decrease of SOD activity. The results of L16(2×42) orthogonal test of high and low temperature synergy showed that SOD relative enzyme activity was significantly increased by 54.6% after treatment at 30°C for 2 h and 10°C for 1.5 h, but the order of high and low temperature treatment had no effect on SOD activity. difference. Ultrasonic experiments showed that the SOD activity was increased by 41.9% compared with the control when the ultrasonic wave was treated at 38 kHz for 40 min, and the enzyme activity was significantly decreased after prolonged treatment time. The results showed that short-term physical stress, such as high and low temperature stress, co-stress, and ultrasonic treatment, could increase the SOD activity of Yunzhi mycelia at a certain stress intensity and within a certain processing time range. The high and low temperature synergistic stress increased. The effect of SOD activity is more pronounced than monotemperature treatment.