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目的建立沈阳红药胶囊中羟基红花黄色素A含量测定的方法。方法色谱柱为Agilent Eclipse XDB C18(250mm×4.6 mm,5μm);以甲醇-乙腈-0.7%的磷酸溶液(26:2:72)为流动相;流速1.0m L/min;检测波长403nm;柱温30℃。结果羟基红花黄色素A质量浓度在9.96~159.36μg/ml范围内与峰面积呈良好线性关系(r=0.9992),加样回收率为99.11%,RSD为0.96%(n=6);结论本方法简便、准确、重复性好,可用于沈阳红药胶囊中羟基红花黄色素A的含量测定。
Objective To establish a method for determination of hydroxysafflor yellow A in Shenyang Hongyao Capsule. Methods The column was Agilent Eclipse XDB C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-acetonitrile-0.7% phosphoric acid solution (26: 2: 72) Temperature 30 ℃. Results The mass concentration of hydroxysafflor yellow A was linear with the peak area in the range of 9.96-159.36 μg / ml (r = 0.9992). The recovery was 99.11% and the RSD was 0.96% (n = 6). Conclusion The method is simple, accurate and reproducible, and can be used for the determination of hydroxysafflor yellow A in Shenyang Hongyao Capsule.