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目的:探讨大分子葡萄糖酐血瘀证大鼠表征的偶氮蓝变化特征与血液流变性的相关性。方法:分别对大鼠尾iv0.5%偶氮蓝配制的10%大分子葡萄糖酐溶液,0.5%偶氮蓝溶液,生理盐水配制10%的大分子葡萄糖酐溶液,或生理盐水,在注射后30,60,120,240min观察和拍摄大鼠耳廓、舌体表征变化,检测血液流变性,分析体表特征变化与血液流变学指标间的相关性。结果:①尾iv0.5%偶氮蓝配制的10%大分子葡萄糖酐溶液的大鼠30min时耳廓和舌出现蓝色斑点,并随时间延长,蓝色瘀斑范围增大,240min时耳廓完全呈深蓝色,舌尖蓝色瘀斑范围继续增加,几乎占舌体1/2;而只注射0.5%偶氮蓝的大鼠对照组耳廓始终半透明,舌体颜色均匀。②iv生理盐水配制10%的大分子葡萄糖酐溶液的大鼠与只注射生理盐水的大鼠相比,30,60min不同减切速率下全血黏度均显著增高,30,60,120,240min血浆黏度、红细胞聚集指数、聚集指数积分也均显著增高,而30,60,120,240min的红细胞变形面积指数、变形指数积分面积虽未测出明显差异,但趋势表现为降低。结论:大鼠尾iv0.5%偶氮蓝配制的10%大分子葡萄糖酐后能使血瘀证模型大鼠的血瘀程度在其耳廓和舌体表征中较为直观地呈现,且与注射大分子葡萄糖酐后血液流变性变化所反映的血瘀程度相一致,并呈明显的时效关系,进一步说明偶氮蓝可以客观反映大分子葡萄糖酐所致血瘀证模型大鼠血瘀程度的表征变化。
Objective: To investigate the correlation between azo blue changes and blood rheology in macromolecular glucose-induced blood stasis syndrome rats. Methods: 10% macromolecule dextran solution, 0.5% azo blue solution and 10% macromolecule dextran solution were prepared respectively from rat tail iv 0.5% azo blue. After injection 30,60,120,240 min observation and filming the auricle, tongue changes in the rat, the detection of hemorrheology, analysis of changes in body surface characteristics and the correlation between hemorheological parameters. Results: (1) At 30 minutes after caudal iv 0.5% azo blue solution of 10% macromolecule glucose solution, blue spots appeared on the auricle and tongue of the rats, and the area of blue ecchymosis increased with the passage of time. The outline was completely dark blue, the scope of tongue blue ecchymosis continued to increase, accounting for almost 1/2 of the tongue. The auricle of the control group with only 0.5% azo blue injected was translucent and the color of the tongue was uniform. Compared with rats injected with saline only, the viscosity of whole blood at 30,60 and 60 min was significantly increased, the plasma viscosity at 30, 60, 120 and 240 min, the aggregation of erythrocytes Index and aggregation index were also significantly increased, while 30,60,120,240 min erythrocyte deformation area index, although the deformation index integral area was not significantly different, but the trend showed a decrease. CONCLUSION: The 10% macromolecules of glucose anhydroglucose prepared from caudal iv 0.5% azobenzene can make the blood stasis of the rats with blood stasis syndrome more intuitive in the appearance of auricle and tongue, The change of blood rheology of macromolecule after glucose is consistent with the degree of blood stasis, and showed a significant time-dependent relationship, further indicating that azo blue can objectively reflect the macromolecule glucose-induced blood stasis syndrome in rats model of blood stasis Variety.