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目的:建立测定口服补液盐散(Ⅰ)中葡萄糖含量的方法。方法:采用高效液相色谱-示差折光检测法。色谱柱为Hypersil APS-2(NH2)柱,流动相为乙腈-水(75:25),流速为1.0mL.min-1,柱温为40℃,检测器温度为40℃,进样量为20μL。结果:葡萄糖检测浓度在0.2082～2.082mg.mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均回收率为101.40%,RSD=2.00%(n=9)。结论:该方法操作快速、简便,专属性好,耗样量少,结果准确、可靠,适用于口服补液盐散(Ⅰ)中葡萄糖的含量测定。
Objective: To establish a method for determination of glucose in oral rehydration salt (Ⅰ). Methods: High performance liquid chromatography - refractive index detection. The column was Hypersil APS-2 (NH2) column. The mobile phase was acetonitrile-water (75:25), the flow rate was 1.0mL.min-1, the column temperature was 40 ℃, the detector temperature was 40 ℃, 20 μL. Results: The concentration of glucose in the range of 0.2082 ~ 2.082mg.mL-1 showed a good linear relationship with the peak area (r = 0.9999). The average recovery was 101.40% and RSD was 2.00% (n = 9). Conclusion: The method is rapid, simple and convenient, has good specificity, less sample consumption, accurate and reliable results and is suitable for the determination of glucose in oral rehydration salt.