肉毒梭菌芽胞广泛分布于自然界,在芽胞分布密度较高的地区,一些农产品、海产品、蔬菜、水果等常被污染,当芽胞遇有适宜的生长条件,即能发芽增殖而产毒,在食入含有肉毒毒素的食品后,则可引起肉毒食物中毒。食品中肉毒梭菌的污染来源主要是土壤,因此,调查肉毒梭菌及其菌型在自然环境中的分布状况,对于肉毒中毒的防治具有重要的意义。多年来对肉毒中毒的检验主要靠动物毒性试验法(以下简称常规法)做最后判定,此法 Clostridium botulinum spores are widely distributed in nature, sprouts in the distribution of high density areas, some agricultural products, seafood, vegetables, fruits and other often polluted, when the spores meet the appropriate conditions for growth, that can germinate and produce poison, Ingestion of foods containing botulinum toxin, it can cause botulism poisoning. Clostridium botulinum pollution in food is mainly soil, therefore, to investigate the distribution of Clostridium botulinum and its bacteria in the natural environment for the prevention and treatment of botulism is of great significance. Botulinum toxicity over the years mainly by animal toxicity test (hereinafter referred to as the conventional method) to make the final decision, this method