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目的:观察黄芪注射液对2型糖尿病动物模型KKAy小鼠脑微血管病变的影响,探讨黄芪注射液对糖尿病脑血管病变的保护作用。方法:饲养至14周龄的雄性KKAy小鼠随机分成模型组和黄芪注射液治疗组(每日腹腔给药,剂量为3 mL/kg),同龄雄性C57BL/6J小鼠作为对照组。血糖仪测量20、24、28周龄时各组小鼠的空腹血糖水平。28周龄时处死各组小鼠,放射免疫法检测血清6-酮-前列腺素-F1(α6-Keto-PGF1α)和血栓素B(2TXB2)的含量。透射电子显微镜观察脑组织超微结构变化。结果:模型组KKAy小鼠从20周龄开始血糖水平明显高于正常组小鼠(P<0.01);黄芪治疗组小鼠从20周龄开始血糖水平明显高于正常组小鼠(P<0.01),但低于模型组小鼠(P<0.05或P<0.01)。模型组小鼠血清6-Keto-PGF1α水平较正常组降低(P<0.01),TXB2含量增高(P<0.01);与模型组相比,黄芪注射液治疗组小鼠6-Keto-PGF1α水平升高(P<0.01),TXB2含量下降(P<0.01)。透射电镜显示模型组小鼠神经细胞胞核染色质疏松,线粒体肿胀,粗面内质网缩小,核糖体减少;治疗组小鼠以上病变明显改善。结论:黄芪注射液可以有效改善2型糖尿病动物模型KKAy小鼠脑微血管病变,保护神经细胞结构。
Objective: To observe the effect of astragalus injection on brain microangiopathy in KKAy mice with type 2 diabetes mellitus, and to explore the protective effect of astragalus injection on diabetic cerebral vascular lesions. Methods: Male KKAy mice fed to 14 weeks of age were randomly divided into model group and astragalus injection group (daily intraperitoneal injection at a dose of 3 mL / kg), and male C57BL / 6J mice of the same age served as control group. Blood glucose was measured at 20,24,28 weeks of age when the fasting blood glucose levels of mice in each group. At 28 weeks of age, mice in each group were sacrificed and the levels of serum 6-keto-PGF1α and 2TXB2 were measured by radioimmunoassay. Transmission electron microscopy was used to observe the ultrastructural changes of brain tissue. Results: The blood glucose level of KKAy mice in model group was significantly higher than that in normal mice from the age of 20 weeks (P <0.01), and the blood glucose level in astragalus mongholicus group from 20 weeks old was significantly higher than that in normal mice (P <0.01) ), But lower than the model mice (P <0.05 or P <0.01). The level of 6-Keto-PGF1α in the model group was lower than that in the normal group (P <0.01) and TXB2 content was increased (P <0.01). Compared with the model group, the level of 6-Keto- TXB2 content decreased (P <0.01). Transmission electron microscopy showed that in the model group, the chromatin in the nucleus of the neurons was loose, the mitochondria were swollen, the rough endoplasmic reticulum was reduced and the ribosomes were reduced. The lesions in the treated group were significantly improved. Conclusion: Astragalus injection can effectively improve the brain microangiopathy in KKAy mice, an animal model of type 2 diabetes, and protect the structure of nerve cells.