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目的测定不同饮水型砷暴露人群血液雌激素受体β(ERβ)mRNA表达水平,分析ERβmRNA表达水平与砷致心脏损伤的关系,进一步探讨砷的内分泌干扰效应。方法采用分子流行病学调查方法,在饮水型地方性砷中毒病区选择调查对象273人,依据饮水砷浓度分为高、中、低和对照4个剂量组,运用实时荧光定量RT-PCR技术,检测其血液ERβmRNA表达水平。结果人群ERβmRNA表达量随着饮用水砷浓度及尿砷含量的增加而上升(r分别为0.159和0.21,P<0.05);随水砷浓度的增加Q-Tc间期延长患病率上升(χ2=4.35,P=0.037),各组ERβmRNA表达水平与Q-Tc间期延长患病率变化趋势一致;Tp-Te间期随水砷浓度的增加而延长(r=0.199,P=0.023),同样也随ERβmRNA表达的增加而延长(r=0.205,P=0.019);各组心律失常患病率几乎与ERβmRNA表达水平变化趋势同步。结论长期砷暴露可潜在地影响人体ERβ基因表达,砷致Q-Tc间期和Tp-Te间期延长与干扰ERβ基因表达可能有密切联系。
Objective To determine the mRNA expression level of ERβ in different drinking arsenic-exposed groups, and to analyze the relationship between ERβ mRNA expression and heart damage induced by arsenic, and to further explore the effect of arsenic on endocrine disruption. Methods According to the method of molecular epidemiology, 273 people were selected in endemic arsenism in drinking water. According to the concentration of arsenic in drinking water, the four groups were divided into four groups: high, middle, low and control. Real-time fluorescence quantitative RT-PCR , Detect their blood ERβmRNA expression level. Results The expression of ERβmRNA in the population increased with the increase of drinking water arsenic and urine arsenic (r = 0.159 and 0.21 respectively, P <0.05). The prevalence of Q-Tc prolonged with the increase of arsenic concentration (χ2 = 4.35, P = 0.037). The prevalence of ERβmRNA expression in all groups was consistent with the prolongation of Q-Tc interval. The Tp-Te interval was prolonged with the increase of arsenic concentration (r = 0.199, P = 0.023) (R = 0.205, P = 0.019). The prevalence of arrhythmia in each group was almost the same as that of ERβmRNA expression. Conclusion Long-term exposure to arsenic can potentially affect the expression of ERβ gene in human. The prolongation of arsenic induced Q-Tc interval and Tp-Te interval may be closely related to the interference of ERβ gene expression.