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目的 探讨Bmi-1分子在直肠癌组织中的达及其临床意义.方法 选取由临床结直肠癌患者癌组织和癌旁组织构建的组织芯片,采用免疫组化法分析Bmi-1分子在结直肠癌组织中的表达,应用Aperio病理扫描系统采集图像,进而由两位病理科医师读片分析.采用Kaplan-Meier法进行生存期分析.结果 Bmi-1分子高表达于结直肠癌组织细胞核;淋巴结转移组Bmi-1分子表达高于未转移组(P＝0.026)、结直肠癌临床Ⅲ期和Ⅳ期Bmi-1分子表达高于临床Ⅰ期和Ⅱ期(P＝0.013);不同年龄、性别及结直肠癌病理分级组别肠癌组织中Bmi-1分子的表达差异无统计学意义(P＞0.05);Bmi-1高表达组患者的总生存期明显低于Bmi-1低表达组(P＜0.01).结论 Bmi-1分子高表达于结直肠癌组织细胞核,与大肠癌的转移和患者生存期密切相关,可能是结直肠癌临床病理诊断的标记分子和预后指标.“,”Objective To explore the expression of Bmi-1 on colorectal cancer tissue and its clinical significance. Methods Tissue chip that contains the tumor tissue and paracancerous tissue from colorectal cancer patients was applied to analyze Bmi-1 expression by immunohistochemical staining. And then the tissue chip was scanned with Aperio pathological scan system and diagnosed by 2 experienced pathologists. Results Bmi-1 expressed highly in the nuclear of tumor tissue from colorectal cancer patients. Bmi-1 expression in tumor tissues from lymph node metastasis patients is higher than that from group without lymph node metastasis (P＝0.026). Bmi-1 expression in tumor tissues from patients with clinical stage Ⅲ and Ⅳ is higher than that from patients with stage Ⅰ and Ⅱ(P＝0.013). There is no significant difference of Bmi-1 expression in tumor tissues from patients with different pathological grading, age and gender (P＞0.05). Kaplan-Meier analysis showed that over-expression of Bmi-1 predicted shorter overall survival. Conclusion Bmi-1 highly expressed in the nuclear of tumor tissue from colorectal cancer patients and closely correlated with metastasis and survival. Bmi-1 may be an indicator of pathological diagnostic and prognostic indicator of colorectal cancer.