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目的:观察Ras蛋白激活物类似物-1(Ras protein activator like 1,RASAL1)蛋白在食管鳞癌组织中的表达情况及其与肿瘤病理分级的相关性,探讨其与食管鳞癌发生、发展的关系。方法:应用组织芯片技术及免疫组织化学实验检测92例食管鳞癌组织及其癌旁食管黏膜组织中RASAL1蛋白的表达,采用LeicaQ550图像分析仪定量分析免疫组化结果。结果:免疫组织化学实验结果表明,病理分型Ⅰ型、Ⅱ型、Ⅲ型食管鳞癌组织中RASAL1蛋白的表达强度较癌旁食管正常黏膜组织明显下降,且癌组织中RASAL1蛋白表达下降程度与肿瘤分化程度呈一致趋势。计算机图像分析结果显示,与肿瘤周围正常对照组织相比,Ⅰ型和Ⅲ型分别下降了74.9%和93.8%,统计学检验结果显示差异均有统计学意义(P均<0.01)。结论:RASAL1蛋白的表达下调与食管鳞癌的发生、发展密切相关,且可能与食管鳞癌的病理分型有关。
OBJECTIVE: To observe the expression of Ras protein activator like 1 (RASAL1) in esophageal squamous cell carcinoma (ESCC) and its correlation with pathological grade of esophageal squamous cell carcinoma relationship. Methods: The expression of RASAL1 protein was detected in 92 esophageal squamous cell carcinomas and adjacent non-cancerous esophageal mucosa tissues by tissue microarray and immunohistochemistry. The results of immunohistochemistry were analyzed by Leica Q550 image analyzer. Results: The results of immunohistochemistry showed that the expression of RASAL1 protein in pathological type Ⅰ, type Ⅱ and type Ⅲ esophageal squamous cell carcinoma tissues was significantly lower than that in normal esophageal mucosa, and the decrease of RASAL1 protein expression Tumor differentiation showed a consistent trend. Computer image analysis showed that the type Ⅰ and type Ⅲ were decreased by 74.9% and 93.8% respectively compared with the normal control tissues around the tumor, and the statistical test results showed that the difference was statistically significant (all P <0.01). Conclusion: The down-regulation of RASAL1 protein is closely related to the occurrence and development of esophageal squamous cell carcinoma and may be related to the pathological type of esophageal squamous cell carcinoma.