论文部分内容阅读
目的探讨中风醒脑液对大鼠脑缺血-再灌注损伤脑组织的保护作用。方法36只大鼠建立大鼠右侧大脑中动脉缺血-再灌注损伤模型后均分为两组,A组腹腔注射依达拉奉注射液3mg/kg,B组予中风醒脑液灌胃,每日3次,每次5ml。另取18只大鼠腹腔注射生理盐水作为假手术对照(C组)。麻醉苏醒后进行神经功能缺损评分(NFDS),取脑组织行TTC染色比较脑梗死体积,ELISA法检测脑组织TNF-α及IL-10水平。结果 B组神经功能缺损评分低于A组[(1.87±0.56)分vs.(2.56±0.99)分](P<0.05)。B组组脑梗死体积小于A组[(98.7±1.5)mm~3 vs.(103.6±2.4)mm~3](P<0.05)。与C组比较,A、B组大鼠脑组织IL-10及TNF-α水平升高(P<0.01),B组IL-10及TNF-α水平低于A组(P<0.01)。结论中风醒脑液对脑缺血-再灌注损伤大鼠脑组织有保护作用,其机制可能与其抗炎作用相关。
Objective To investigate the protective effect of Zhongfeng Xingnao Liquid on brain tissue after cerebral ischemia-reperfusion injury in rats. Methods Thirty-six rats were divided into two groups according to the model of right middle cerebral artery occlusion (MCAO). Group A received intradermal injection of 3 mg / , 3 times a day, each 5ml. Another 18 rats intraperitoneal injection of saline as a sham control (C group). Nerve function deficit score (NFDS) was obtained after anesthesia awakening. Brain infarct volume was determined by TTC staining. The levels of TNF-α and IL-10 in brain tissue were measured by ELISA. Results The score of neurological deficit in group B was lower than that in group A [(1.87 ± 0.56) vs (2.56 ± 0.99)] (P <0.05). The volume of cerebral infarction in group B was smaller than that in group A [(98.7 ± 1.5) mm ~ 3 vs. (103.6 ± 2.4) mm ~ 3] (P <0.05). Compared with group C, the level of IL-10 and TNF-α in group A and group B were increased (P <0.01), while the level of IL-10 and TNF-α in group B was lower than that in group A (P <0.01). Conclusion Stroke Xingnao liquid has a protective effect on brain tissue of rats with cerebral ischemia-reperfusion injury, and its mechanism may be related to its anti-inflammatory effect.