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目的研究杭州湾南岸滩涂沉积物中POPs混合物对小鼠的生殖毒性作用。方法选择健康清洁级性成熟ICR小鼠80只,体重22~26 g,随机分为30、100、300 mg/kg三个剂量染毒组和一个溶剂对照组,每组20只,雌雄各半,进行一代生殖毒性试验。采用灌胃方式进行染毒(10 ml/kg),雄鼠染毒6周,进行精子畸形测定。雌鼠染毒2周后,雌雄小鼠按1:1同笼交配,交配时间为2周,观察交配率及受孕率。孕鼠在妊娠19 d解剖,观察黄体数、着床数、活胎数、死胎数、吸收胎数、每窝胎仔平均胎长、胎重、胎盘重。结果当混合物染毒剂量为100 mg/kg和300 mg/kg时,精子畸形率明显升高(P<0.05),剂量达到300 mg/kg时生育率、妊娠率、活胎率、平均胎长和胎仔重降低(P<0.05),而着床前死亡率、死胎率升高(P<0.05)。结论滩涂沉积物中POPs混合物在剂量达到300 mg/kg时对小鼠产生明显的生殖和发育毒性。
Objective To study the reproductive toxicity of POPs mixture in beach sediments of the south bank of Hangzhou Bay. Methods Totally 80 mice with healthy and clean grade mature ICR mice weighing 22-26 g were randomly divided into three dose groups of 30, 100, and 300 mg / kg and a solvent control group, with 20 mice in each group, male and female , A generation of reproductive toxicity test. The rats were injected intragastrically (10 ml / kg) for 6 weeks, and sperm deformity was measured. Two weeks after the female mice were exposed to the virus, the male and female mice were mated with 1: 1 cage at the mating time of 2 weeks to observe the mating rate and the pregnancy rate. The pregnant rats were dissected on the 19th day of gestation, and the number of luteal cells, the number of implants, the number of live births, the number of stillbirths, the number of fetuses absorbed, the average fetal length per litter, the weight of the placenta and the weight of the placenta were observed. Results The rate of sperm deformity was significantly increased at 100 mg / kg and 300 mg / kg of the mixture (P <0.05). The fertility rate, pregnancy rate, live birth rate, average fetal length And fetal weight (P <0.05), while the pre-implantation mortality and the stillbirth rate increased (P <0.05). Conclusion The POPs mixture in tidal flat sediments produced obvious reproductive and developmental toxicity in mice when the dose reached 300 mg / kg.