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本研究以山竹不定胚诱导的无菌苗叶片为外植体,通过优化山竹叶片诱导再生的培养条件,建立了山竹再生培养技术体系方法。结果显示,叶片转入WPM+1.0 mg?L~(-1) GA3+1.0 mg?L~(-1) 6-BA+0.5 mg?L~(-1) TDZ培养基中,诱导结节性愈伤组织的比例为62.4%。转入添加0.7 mg?L~(-1) TDZ+0.5 mg?L~(-1) 6-BA的WPM胚性愈伤组织诱导培养基中,诱导出36%的球状胚性愈伤组织。8周后球状胚性愈伤组织转入不定芽诱导培养,在含有WPM+2.0 mg?L~(-1) 6-BA+0.5 mg?L~(-1) NAA的培养基中,增殖系数达12.54。当不定芽长到20 mm左右时,转接到生根培养基中进行生根诱导,诱导生根的最适培养基为1/2MS+0.5 mg?L~(-1)6-BA+0.5 mg?L~(-1) NAA+0.1 mg?L~(-1) IBA,生根率为85.2%。
In this study, aseptic shoots induced by adventitious buds of mangosteen were used as explants. By optimizing the culture conditions of regenerating mangosteen leaves, a method system of mangosteen regeneration culture was established. The results showed that the leaves were transferred into WPZ + 1.0 mg? L -1 GA 3 + 1.0 mg? L -1 (-1) 6-BA + 0.5 mg? L -1 TDZ medium to induce nodularity The callus was 62.4%. Transferred into WPM embryogenic callus induction medium supplemented with 0.7 mg? L -1 TDZ + 0.5 mg? L? -1-BA and induced 36% globular embryogenic callus. After 8 weeks, the globular embryogenic callus was transformed into adventitious buds and cultured in medium containing WPM + 2.0 mg? L-6-BA + 0.5 mg? L -1 NAA. The proliferation coefficient Up to 12.54. When adventitious buds grow to about 20 mm in length, the optimal medium for rooting is 1 / 2MS + 0.5 mg? L-6-BA + 0.5 mg? L ~ (-1) NAA + 0.1 mg? L ~ (-1) IBA, the rooting rate was 85.2%.