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目的:模拟中药汤剂微粒体系,探讨不同粒径的壳聚糖粒子(CS-Ps)对中药活性成分小檗碱在体肠吸收特性的影响。方法:采用高压静电喷雾法制备不同粒径壳聚糖粒子,检测粒子粒径与Zeta电位。利用在体肠灌流模型考察壳聚糖粒子粒径特征对小檗碱肠吸收速率常数及表观渗透系数的影响。结果:CS-P_1,CS-P_2,CS-P_3粒径分别为(163.9±2.3),(456.0±76.6),(903.4±131.1)nm,Zeta电位依次为(25.2±7.0),(27.9±4.3),(28.4±5.7)m V,各组粒子间表面电位无显著性差异。与空白组比较,CS-P_1和CS-P_2组粒子对小檗碱肠吸收具有显著的促进作用(P<0.05),CS-P_3组则无显著的促进作用。结论:壳聚糖粒子对小檗碱肠吸收具有促进作用,这种吸收促进作用与壳聚糖粒子的粒径相关。
OBJECTIVE: To simulate the Chinese medicine decoction microparticle system and investigate the effect of different size chitosan particles (CS-Ps) on the intestinal absorption of Berberine, a traditional Chinese medicine active ingredient. Methods: Chitosan particles with different particle size were prepared by high-pressure electrostatic spraying. The particle size and Zeta potential were measured. Effects of chitosan particle size characteristics on intestinal absorption rate constant and apparent permeability coefficient of berberine were investigated in a model of intestinal perfusion. RESULTS: The diameters of CS-P_1, CS-P_2 and CS-P_3 were (163.9 ± 2.3) and (456.0 ± 76.6) and (903.4 ± 131.1) nm, respectively. The Zeta potentials were (25.2 ± 7.0) and ), (28.4 ± 5.7) m V, there was no significant difference in the surface potential between the groups. Compared with the blank group, the CS-P 1 and CS-P 2 groups had significant effects on the intestinal absorption of berberine (P <0.05), but no significant promotion in the CS-P 3 group. CONCLUSION: Chitosan particles can promote intestinal absorption of berberine, and this absorption-promoting effect is related to the particle size of chitosan particles.