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目的:观察加味桃红四物汤含药血清对体外培养的动脉粥样硬化血瘀证大鼠血管内皮细胞(VECs)的保护作用及其机制。方法:制备加味桃红四物汤含药血清及动脉粥样硬化血瘀证模型大鼠,从模型大鼠胸主动脉分离培养VECs,将VECs分为对照组、血瘀血清组、中药血清组分别进行处理,采用改良的Boyden chamber法观察VECs迁移的活性,硝酸还原酶法测定培养液中NO含量,ELASA法测定培养液中ET、t-PA/PAI-1、ICAM-1含量,RT-PCR检测eNOSmRNA、ICAMmRNA的表达。结果:血瘀血清组培养液中NO、t-PA含量低于对照组与中药血清组(P<0.05),ET、PAI-1、ICAM-1含量高于对照组与中药血清组(P<0.05);VECs经血瘀血清处理后,迁移活性降低,而经中药血清干预后,VECs迁移活性增加,与血瘀血清组比较,差异有统计学意义(P<0.05)。血瘀血清组eNOSmRNA表达低于中药血清组,而ICAMmRNA表达高于中药血清组。结论:加味桃红四物汤药物成分可促进动脉粥样硬化血瘀证大鼠VECs迁移,并促进VECs保护性因子表达。
Objective: To observe the protective effect and mechanism of Jiawei Taohongsiwutang medicated serum on vascular endothelial cells (VECs) cultured in vitro in atherosclerotic blood stasis rats. Methods: Modified Taohongsiwutang medicated serum and atherosclerosis blood stasis syndrome rat model were isolated and cultured from the thoracic aorta, VECs were divided into control group, blood stasis serum group, Chinese medicine serum group The contents of ET, t-PA / PAI-1 and ICAM-1 in the culture medium were measured by ELASA, and the content of ICAM-1 in the culture fluid was determined by RT-PCR Detection of eNOS mRNA, ICAM mRNA expression. Results: The content of NO and t-PA in the blood stasis group was lower than that in the control group and the Chinese medicine serum group (P <0.05), and the content of ET, PAI-1 and ICAM-1 in the blood stasis group was higher than that in the control group and the TCM serum group 0.05). After VECs were treated with blood stasis serum, the migration activity of VECs decreased. However, migration of VECs increased with the intervention of Chinese medicine serum. Compared with blood stasis serum group, the difference was statistically significant (P <0.05). The expression of eNOS mRNA in blood stasis group was lower than that in traditional Chinese medicine group, while the expression of ICAM mRNA was higher than that in traditional Chinese medicine group. Conclusion: Modified Taohong Siwu decoction can promote the migration of VECs in atherosclerotic blood stasis rats and promote the expression of protective factor in VECs.