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新生小牛肝经组织匀浆、加热和离心制得粗制的肝细胞生长因子（ＨＧＦ），然后经过超滤、ＤＥＡＥＤＥ５２纤维层析，Ｓｕｐｅｒｏｓｅ１２凝胶过滤，ＭｏｎｏＱ离子交换层析和高效液相色谱进行分离提纯，用３Ｈ标记的胸腺嘧啶核苷掺入法在人肝瘤ＳＭＭＣ７２２１细胞株上检测提纯的新生小牛肝细胞生长因子的生物学活性，得到一分子量为（１２～１４）×１０３的有促进肝细胞有丝分裂的有效成分。显而易见此ＨＧＦ的分子量不同于循环在血浆中的ＨＧＦ的分子量。本文对２种ＨＧＦ的不同作用进行了讨论。
Neonatal calf liver tissue homogenate, heating and centrifugation obtained crude hepatocyte growth factor (HGF), and then after ultrafiltration, DEAE DE52 fiber chromatography, Superose12 gel filtration, MonoQ ion exchange chromatography and high performance liquid Phase chromatographic separation and purification, with 3H-labeled thymidine incorporation assay in human hepatoma SMMC 7221 cell line purified purified calf hepatocyte growth factor biological activity obtained a molecular weight (12 ~ 14) × 103 has the promotion of hepatocyte mitosis of the active ingredient. It is obvious that the molecular weight of this HGF is different from the molecular weight of HGF circulating in plasma. This article discusses the different roles of the two HGFs.