论文部分内容阅读
目的探讨慢性阻塞性肺疾病(COPD)患者肺组织中血管内皮生长因子(VEGF)、诱生型一氧化氮合酶(iNOS)的表达情况及吸烟对二者表达的影响。方法取46例因肺癌行肺叶切除患者的癌旁组织,依据吸烟及肺功能情况分成:(1)吸烟伴 COPD 组19例;(2)吸烟不伴 COPD 组12例;(3)不吸烟不伴 COPD 组15例。用免疫组化法检测肺组织 VEGF 及 iNOS 的表达。结果吸烟不伴COPD 组(1.50±0.39,1.45±0.41)与不吸烟不伴 COPD 组(1.18±0.33,1.09±0.41)比较,肺组织VEGF、iNOS 表达增强;吸烟伴 COPD 组(2.19±0.51,2.39±0.45)与不吸烟不伴 COPD 组比较表达明显增强。肺组织 VEGF 表达与 iNOS 表达呈明显正相关(r=0.78,P<0,01)。肺组织 VEGF 表达与FEV_1呈明显负相关(r=-0.67,P<0.01)。结论吸烟以及轻度 COPD 患者肺组织 VEGF、iNOS 表达上调,iNOS、VEGF 的过度表达可能参与 COPD 患者气道与血管重建和气流受限的过程。
Objective To investigate the expression of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase (iNOS) in lung tissue of patients with chronic obstructive pulmonary disease (COPD) and the effect of smoking on their expression. Methods According to smoking and pulmonary function, 46 patients with lung cancer undergoing lobectomy were divided into (1) smoking with COPD group (19); (2) smoking without COPD group (12); (3) without smoking With COPD group of 15 cases. Immunohistochemistry was used to detect the expression of VEGF and iNOS in lung tissue. Results Compared with COPD group (1.50 ± 0.39, 1.45 ± 0.41) and non-smoking non-smoking COPD group (1.18 ± 0.33, 1.09 ± 0.41), the expressions of VEGF and iNOS in lung tissues were significantly increased in smokers with COPD group (2.19 ± 0.51, 2.39 ± 0.45) and non-smoking without COPD group was significantly increased expression. Lung tissue VEGF expression and iNOS expression was positively correlated (r = 0.78, P <0,01). The expression of VEGF in lung tissue was negatively correlated with FEV_1 (r = -0.67, P <0.01). Conclusions The expressions of VEGF and iNOS in lung tissue of smokers and mild COPD patients are up-regulated. Overexpression of iNOS and VEGF may be involved in airway and vascular remodeling and airflow limitation in COPD patients.