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目的:观察益气健脾法代表方剂对实验性脾虚证大鼠模型红细胞膜腺苷三磷酸酶 (ATPase)活性的影响.方法:取SD大鼠60只随机分为6组,即正常对照组、脾虚模型组、四君子汤组、当归补血汤组、黄芪四君子汤组、当归补血汤合四君子汤组,采用破气苦降加饥饱失常法复制脾虚证大鼠模型,用定磷法检测大鼠红细胞膜ATPase的活性.结果:益气健脾方药能显著升高脾虚证大鼠模型红细胞膜ATPase的活性.结论:从微观的角度验证了中医“脾主运化”的理论.“,”Objective:To observe the effect of Qi Activating and spleen - strengthening Therapy on the ATPase activity in red cell envelope of rats with spleen deficiency system.Methods:Sixty rats were randomly devided into 6 groups:normal group,model group,SiJunZi Decoction(SJD) group,DanguiBuXue Decoction(DBD) group,HuangQiSiJunzi Decoction (HSJD) group,and DBD + SJD group.The rat model of spleen deficiency was built by Qibreaking and bitter taste herbs and alternation of starvation and gorge.Determining - phosphorus method was used to detect the ATPase activity of red cell envelope of rats.Results:Qi activating and spleen strengthening herbs could increase the ATPase activity in red cell envelope of rats with spleen deficiency syndrome obivously.Conclusions:The study verified the theory of TCM that spleen dominates digestion from microcosmic angle.