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以甜瓜抗白粉病品种‘银帝’和感白粉病品种‘卡拉克赛’为材料,研究诱导抗性处理对其叶片酚类物质代谢的影响及其与抗病性的关系。结果表明:苯丙噻重氮(BTH)和水杨酸(SA)处理以及接种白粉菌均可显著诱导甜瓜叶片苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)活性和绿原酸、总酚、类黄酮含量升高,但纳米硅(SiO2)诱导无效。经诱导和接菌后,甜瓜抗病品种叶片PAL、POD、PPO活性和总酚、绿原酸、类黄酮含量高于感病品种。BTH和SA诱导甜瓜抗病性增强的原因之一是提高了叶片次生代谢相关酶的活性,增加了叶组织次生代谢物质的含量。
The melon resistant powdery mildew variety ’Silver Emperor’ and susceptible powdery mildew variety ’Kracai’ were used to study the effects of induced resistance on the phenolic metabolism in leaves and their relationship with disease resistance. The results showed that both benzathine diazoxide (BTH) and salicylic acid (SA) treatment and powdery mildew inoculation could significantly induce PAL, POD and polyphenol Oxidase (PPO) activity and chlorogenic acid, total phenol, flavonoid content increased, but the silicon dioxide (SiO2) induced ineffective. After induction and inoculation, the activities of PAL, POD and PPO and the contents of total phenolic, chlorogenic acid and flavonoids in resistant varieties of melon were higher than those of susceptible varieties. One of the reasons why BTH and SA induced the disease resistance of muskmelon was to increase the activity of enzymes related to secondary metabolism and to increase the content of secondary metabolites in leaf tissue.