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Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers.Thus,the present study prepared amyloid-beta 42 oligomers and constructed a na?ve human Fab phage display library based on blood samples from six healthy people.After three rounds of biopanning in vitro,a human single-domain antibody that specifically recognized amyloid-beta 42 oligomers was identified.Western blot and enzyme-linked immunosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer,but not the monomer or high molecular weight oligomers.This study successfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.
Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oligomers and constructed a naïve human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that specifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked immunosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study successfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.