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建立了反相高效液相色普法测定血浆中莫雷西嗪浓度。采用内标法。流动相为甲醇－水－０.0065mol.L~(-1)醋酸铵－冰乙酸（７５：２４：０.０１５，v/v/v/v），二氯甲烷提取。血药浓度在５０～５０００ng.mL~(-1)范围内呈线性关系，相关系数0.9997，血浆最低检测浓度４ng.mL~(-1).。方法回收率９６～１０２％，日内、日间ＲＳＤ为０.7～3.2和２.3~4.0％。应用该法研究了兔静脉注射药动学，用二室模型拟合，消除相半衰期为6.35±2.23h。本法简便、回收率和灵敏度高、重复性好，分析周期短，适于临床药代动力学和药效学的研究。
A reversed phase high performance liquid chromatography was established for the determination of plasma concentration of. Using internal standard method. The mobile phase was methanol-water-0.0065mol.L -1 ammonium acetate-glacial acetic acid (75:24: 0.015, v / v / v / v) and extracted with methylene chloride. The plasma concentration was linear in the range of 50 ~ 5000 ng.mL ~ (-1) with a correlation coefficient of 0.9997 and the lowest plasma concentration of 4 ng.mL ~ (-1). The recovery rate was 96% -102%. The intra-day and inter-day RSD were 0.7-3.2 and 2.3-4.0%. This method was used to study the pharmacokinetics of intravenous injection in rabbits and fitted with a two-compartment model to eliminate phase half-life of 6.35 ± 2.23 h. This method is simple, high recovery and sensitivity, good repeatability, short analysis period, suitable for clinical pharmacokinetics and pharmacodynamics research.