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目的评价糖化血红蛋白(HbA1c)即时检验(POCT)法和高效液相色谱(HPLC)法的一致性,探讨POCT法测定HbA1c临床应用的可能性。方法随机选取42例门诊糖尿病患者进行HbA1c的HPLC法和POCT法测定,评价2种方法的一致性和HbA1c POCT法临床应用的可行性。结果对2种测定方法进行配对t检验:不分组、HbA1c≤8.0%组、HbA1c>8.0%组,P值分别为0.226、0.899、0.080,两者之间差异无统计学意义;相关分析显示2种方法相关性良好(P<0.05);Bland-Altman一致性分析显示不分组、HbA1c≤8.0%组、HbA1c>8.0%组分别有97.62%、95.45%、95.00%的点位于界限以内。结论 HPLC法与POCT法检测HbA1c结果一致性好;POCT法能满足临床应用的需求,可以作为门诊快速检测HbA1c的较好方法。
Objective To evaluate the consistency of HbAlc real-time assay (POCT) and high performance liquid chromatography (HPLC), and to explore the possibility of using POCT to determine the clinical application of HbA1c. Methods 42 cases of outpatients with diabetes mellitus were selected for HbA1c by HPLC and POCT method to evaluate the consistency of the two methods and the clinical application of HbA1c POCT. Results Paired t-test was used to test the two methods: no difference, HbA1c≤8.0%, HbA1c> 8.0%, P values were 0.226, 0.899, 0.080, respectively. There was no significant difference between the two methods. (P <0.05). The Bland-Altman concordance analysis showed that there were 97.62%, 95.45% and 95.00% points within the limits of HbA1c≤8.0% and HbA1c> 8.0% respectively. Conclusions HPLC method and POCT method have good agreement with HbA1c. POCT method can meet the needs of clinical application and can be used as a good method for rapid detection of HbA1c in clinic.