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疟原虫子孢子体表覆盖着一种具有高度免疫性的表面环蛋白,它占总蛋白量的10～20%,可能具有保护原虫对抗外界环境的重要功能。研究它的结构显然对了解子孢子表面性质有帮助,并可为制备抗疟原虫感染的疫苗提供依据。但是子孢子不能进行体外培养,由感染蚊体取得其数量又不足以满足需要,故作者参照Ellis等方法筛选出了与该蛋白相应的cDNA阳性克隆,再用Sanger等方法分析了它的基因减基对组成。结果发现此环蛋白的相应基因中存在有以36个减基对为组成单位的十二次重复区,作者由此推论出这个蛋白质具有以12个氨基酸残基
Plasmodium sporozoite body surface covered with a highly immunogenic surface ring protein, which accounts for 10 to 20% of the total protein, may have to protect protozoa against the external environment, an important function. Studying its structure apparently helps to understand the surface properties of sporozoites and provides a basis for the preparation of vaccines against Plasmodium infection. However, sporozoites can not be cultured in vitro, and mosquitoes obtained by the number of infection is not enough to meet the need, so the authors refer to Ellis and other methods to screen out the corresponding cDNA positive clones, and then Sanger and other methods to analyze its gene subtraction Base pair composition. As a result, it was found that a 12-fold repetitive region consisting of 36 subtractive pairs exists in the corresponding gene of this cyclopeptide, and the authors deduced that this protein has a sequence of 12 amino acid residues