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目的：研究枸杞多糖（LBP）体外免疫调节效应的作用机制。方法：以DPH作为荧光深剂，采用荧光偏振法观察LBP对免红细胞膜流动性的影响。并以32P-组蛋白内参入的放射性强度检测法，观察LBP对淋巴细胞胞膜和胞浆蛋白激酶C（PKC）活性的影响。结果：100mg／LLBP可明显促进兔红细胞细胞膜的流动性，并能增强10mg／L和25mg／LConA对膜流动性的促进作用。同时100ms／LLBP尚可增加ConA活化后的小鼠脾淋但细胞膜上的PKC的活性，但对胞浆PKC的活性无影响。结论：LBP的体外作用途径可能是作用于细胞膜，通过促进细胞膜的流动性及促进ConA活化的小鼠脾淋巴细胞胞浆内PKC从胞浆到胞膜的激活移位而发挥免疫调节效应的。
Objective: To study the mechanism of immunomodulatory effects of LBP in vitro. Methods: DPH was used as a deep fluorescent agent. The effect of LBP on the fluidity of red blood cell membrane was observed by fluorescence polarization method. The effect of LBP on the activity of lymphocyte membrane and cytoplasmic protein kinase C (PKC) was measured by radioactive intensity assay of 32P-histone incorporation. RESULTS: 100 mg/L LBP significantly promoted the fluidity of the erythrocyte membrane in rabbits and enhanced the promoting effect of 10 mg/L and 25 mg/L ConA on membrane fluidity. At the same time, 100 ms/LLBP could still increase the activity of PKC on spleen but cell membrane in mice after ConA activation, but had no effect on cytoplasmic PKC activity. Conclusion: The in vitro action pathway of LBP may be on the cell membrane, which exerts the immunomodulatory effect by promoting the fluidity of the cell membrane and promoting the activation of PKC in the cytoplasm of ConA-activated mouse spleen lymphocytes from the cytoplasm to the cell membrane.