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目的 研究口服大剂量米非司酮对子宫内膜癌细胞凋亡和端粒酶活性的影响 ,并探讨其作用机制。方法 12例未治疗的子宫内膜癌患者口服大剂量米非司酮 (10 0mg/d ,共 5天 )。第六天手术 ,用免疫组织化学法(LSAB法 )检测用药前后内膜癌组织Fas、FasL表达的变化。用半定量端粒重复序列扩增法 (semiquantitativetelomererepeatamplificationprotocol,TRAP)测定用药前后内膜癌组织端粒酶活性的改变。 结果 口服大剂量米非司酮后子宫内膜Fas表达明显升高 (P <0 0 1) ,FasL表达及端粒酶活性无明显改变 (P >0 0 5 )。结论 口服大剂量米非司酮可促进子宫内膜癌细胞凋亡 ,但对端粒酶活性无明显影响。
Objective To study the effect of oral high dose mifepristone on apoptosis and telomerase activity of endometrial carcinoma cells and to explore its mechanism. Methods Twelve patients with untreated endometrial cancer were given high-dose mifepristone (10 mg / d for 5 days). On the sixth day of surgery, the expression of Fas and FasL in endometrial carcinoma was detected by immunohistochemistry (LSAB method) before and after treatment. Semiquantitative telomere repeat amplification protocol (TRAP) was used to determine the change of telomerase activity in endometrial carcinoma before and after treatment. Results After oral administration of mifepristone, the expression of Fas in endometrium was significantly increased (P <0.01). The expression of FasL and the activity of telomerase were not significantly changed (P> 0.05). Conclusion Oral administration of mifepristone can promote the apoptosis of endometrial cancer cells, but has no obvious effect on telomerase activity.