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目的：研究胃手术后残胃粘膜癌前期检测方法及用于预测癌变趋势的价值。方法：犬施行Ｂｉｌ－ｒｏｔｈＩＩ式胃手术２月后开始喂饲ＭＮＮＧ，分别于喂饲ＭＮＮＧ第２，３，４，６，８，１０月各时相点进行胃镜检查及粘膜活检，病理学检查及核仁组成区相关嗜银蛋白（ＡｇＮＯＲｓ）染色。结果：从浅表性胃炎到异型增生，ＡｇＮＯＲｓ数目呈递增性增多，颗粒亦渐增大。正常胃粘膜与浅表性胃炎差异不显著（Ｐ＞０．０５），而与萎缩性胃炎、肠上皮化生、异型增生相比有显著差异（Ｐ＜０．０５）。肠上皮化生和异型增生的ＡｇＮＯＲｓ变化在反映细胞增殖、染色体倍数及转录活力方面与其它病变类型相比更具癌前意义。结论：ＡｇＮＯＲｓ作为癌前病变细胞增殖活性及细胞分化异常的指标，具有较好的可靠性和可重复性。可为残胃粘膜癌前期病变的早期检测及癌变趋势的预测提供有价值的依据。
Objective: To study the method of precancerous gastric mucosa detection after gastric surgery and its value in predicting the trend of canceration. Methods: Dogs were fed with Bil-roth II gastric surgery after 2 months and MNNG was fed. Gastroscopy and mucosal biopsy were performed on the 2nd, 3rd, 4th, 6th, 8th and 10th months of MNNG feeding respectively. Pathological examination And nucleolar organizer regions related to silver staining (AgNORs) staining. Results: From superficial gastritis to dysplasia, the number of AgNORs increased gradually and the particles increased gradually. There was no significant difference between normal gastric mucosa and superficial gastritis (P> 0.05), but significant difference compared with atrophic gastritis, intestinal metaplasia and dysplasia (P <0.05). The changes of AgNORs in intestinal metaplasia and dysplasia are more precocious than other types of lesions in reflecting cell proliferation, chromosomal ratio and transcriptional activity. Conclusion: AgNORs as a precancerous cell proliferation activity and abnormal cell differentiation indicators, has good reliability and repeatability. It can provide valuable evidences for the early detection of gastric mucosal precancerous lesions and the prediction of canceration trend.