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为回收利用生产银翘片废弃药渣,以毛头鬼伞为供试菌株,发酵处理银翘药渣;建堆配料设计原则为控制总料C/N接近40∶1,以玉米芯为主料(配合添加奶牛粪、尿素),逐渐增大银翘药渣用量,以替代玉米芯;堆料共采用4个配方,配方A(CK):玉米芯200kg,牛粪100kg,尿素3.5kg,辅料6%(生石灰2%、石膏2%、过磷酸钙2%);配方B:玉米芯100kg,银翘药渣50kg,牛粪50kg,尿素1.0kg,辅料6%;配方C:玉米芯75kg,银翘药渣100kg,牛粪50kg,辅料6%;配方D:玉米芯50kg,银翘药渣150kg,牛粪50kg,辅料6%。4个配方堆料总干质量均在200kg以上,依墙建堆成半圆锥形(h≥1.0m,r≥1.4m);发酵升温14d(4-3-3-2-2)、经4次翻堆后,摊料降温装袋接种,待菌丝满袋后脱袋覆土出菇。结果表明:以配方C的产出率为最高,其相应生产指标为,初始C/N为41.66、终点C/N为23.71、腐熟度T为0.569、接种满袋率93.7%、菌袋平均产量381.9g·kg~(-1)、生物学效率41.0%。经HPLC检测,配方C发酵料中绿原酸、牛蒡苷含量分别为5.20～5.40、4.49～5.30μg·g~(-1),其栽培所得子实体中绿原酸、牛蒡苷含量分别为0.40～0.45、3.81～4.27μg·g~(-1),其中牛蒡苷从栽培料到子实体的迁移率达84.93%。
In order to recycle the waste residues from the production of Yinqiao tablets, the Coprinus comatus was used as the tested strain and the fermented Yinqiao dregs were treated by fermentation. The design principle of the pile-making ingredients was to control the total C / N close to 40: 1 (With the addition of cow dung and urea), gradually increase the amount of Yinqiao dregs to replace the corn cob; stack a total of four formulations, formula A (CK): corn cob 200kg, cow dung 100kg, urea 3.5kg, Formula B: corn cob 100kg, Agkistrodon spill 50kg, cow dung 50kg, urea 1.0kg, auxiliary 6%; formula C: corn cob 75kg , Alice Alice residue 100kg, cow dung 50kg, accessories 6%; formula D: corn cob 50kg, Alice Alice residue 150kg, cow dung 50kg, accessories 6%. The total dry mass of the four formulations was above 200kg, and the reactor was built into a semi-conical wall (h≥1.0m, r≥1.4m). The fermentation temperature was raised for 14 days (4-3-3-2-2) After the second pile, the spread of cold bagging vaccination, mycelium bags to be bagged soil fruiting. The results showed that: the highest yield was in formula C, the corresponding production index was 41.66 for initial C / N, 23.71 for final C / N, 0.569 for maturity, 93.7% for inoculation, and average yield 381.9 g · kg -1, biological efficiency 41.0%. The results of HPLC showed that the contents of chlorogenic acid and arctiin in formula C were 5.20-5.40 and 4.49-5.30μg · g -1, respectively. The content of chlorogenic acid and arctiin in the fruiting body of the recipe C were 0.40 ~ 0.45,3.81 ~ 4.27μg · g ~ (-1), in which the arctiin migration from the material to the fruiting body reached 84.93%.