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目的:建立RP-HPLC法同时测定安神补心片中丹参素钠、红景天苷、2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(以下简称二苯乙烯苷)、女贞子苷、丹酚酸B 5个成分的含量。方法:采用Diamonsil C18色谱柱(4.6 mm×200 mm,5"m)进行分离;流动相为乙腈(A)-0.1%磷酸水溶液(B),梯度洗脱,流速为1.0 mL·min-1;检测波长为220 nm;柱温为30℃。结果:丹参素钠、红景天苷、二苯乙烯苷、女贞子苷、丹酚酸B质量浓度分别在11.94~119.4 mg·L-1(r=0.9994,n=6)、5.520~55.20 mg·L-1(r=0.9997,n=6)、2.848~28.48 mg·L-1(r=0.9992,n=6)、1.104~11.04 mg·L-1(r=0.9990,n=6)、51.78~517.8 mg·L-1(r=0.9993,n=6)与峰面积呈良好的线性关系,方法的平均回收率(n=6)分别为100.1%(RSD=1.8%)、99.7%(RSD=1.8%)、101.1%(RSD=1.2%)、100.8%(RSD=1.8%)和100.1%(RSD=0.9%)。结论:该方法简便、准确,重复性好,专属性强,可用于安神补心片的质量控制。
OBJECTIVE: To establish a RP-HPLC method for the simultaneous determination of sodium salidroside, salidroside, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside Referred to as stilbene glycoside), Ligustriarin, salvianolic acid B 5 components. METHODS: Diamonsil C18 column (4.6 mm × 200 mm, 5 μm) was used for separation. The mobile phase consisted of acetonitrile-A 0.1% phosphoric acid solution (B) with gradient elution at a flow rate of 1.0 mL · min -1 ; The detection wavelength was 220 nm and the column temperature was 30 ℃ .Results: The concentrations of sodium danshensu, salidroside, stilbene glycoside, ligustilide and salvianolic acid B were in the range of 11.94-119.4 mg · L -1 (r = 0.9994, n = 6), 5.520-55.20 mg · L -1 (r = 0.9997, n = 6), 2.848-28.48 mg · L -1 (r = 0.9992, n = 6) and 1.104-11.04 mg The linear relationship was obtained between the peak area and the peak area of L-1 (r = 0.9990, n = 6) and 51.78-517.8 mg · L-1 (r = 0.9993, n = 6) (RSD = 1.8%), 100.1% (RSD = 1.8%) and 100.1% (RSD = 0.9%), respectively. The method is simple, accurate, reproducible and specific, and can be used for quality control of Anshen Buxin tablets.