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目的探讨脂肪乳对三氯乙烯(TCE)诱导的雄性大鼠急性肝损伤干预作用。方法选用5~6周龄无特定病原体级雄性大鼠18只,采用随机数字表法分为对照组、TCE染毒组和脂肪乳干预组,每组6只。TCE染毒组及脂肪乳干预组先予剂量为3 g/kg体质量的TCE一次性灌胃染毒,对照组给予等体积花生油;脂肪乳干预组染毒后立即予质量分数为20.0%脂肪乳(2 mL/kg体质量)一次性经尾静脉注射,TCE染毒组及对照组大鼠经尾静脉注射等体积的0.9%氯化钠溶液。染毒24 h后,大鼠经腹主动脉采血,检测血清丙氨酸氨基转移酶(ALT)及天冬氨酸氨基转移酶(AST)活力;取肝脏组织进行肝脏组织病理学检查,并制备质量分数为10.0%的匀浆检测丙二醛(MDA)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)水平。结果与TCE染毒组比较,脂肪乳干预组肝细胞水肿明显减轻。TCE染毒组大鼠血清中ALT、AST活力以及肝匀浆中MDA、SOD水平均分别高于对照组和脂肪乳干预组(P<0.05);脂肪乳干预组上述4个指标均高于对照组(P<0.05)。3组大鼠肝匀浆中GSH-Px水平比较,差异无统计学意义(P>0.05)。结论脂肪乳可能通过改善脂质过氧化损伤而减轻了TCE诱导的大鼠急性肝损伤。
Objective To investigate the intervention effect of fat emulsion on acute liver injury induced by trichlorethylene (TCE) in male rats. Methods 18 male rats of 5 ~ 6 weeks old without specific pathogen were selected and randomly divided into control group, TCE group and fat emulsion intervention group, with 6 rats in each group. The TCE group and the fat emulsion intervention group were pretreated with TCE at a dose of 3 g / kg body weight, and the control group was given the same volume of peanut oil. The fat emulsion intervention group was given 20.0% fat immediately Milk (2 mL / kg body weight) was injected through the tail vein at one time. TCE-exposed rats and control rats were injected with an equal volume of 0.9% sodium chloride through the tail vein. Twenty-four hours after exposure, blood samples were collected from the abdominal aorta, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity were measured; liver tissues were taken for liver histopathology examination and preparation The content of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected by homogenate with a mass fraction of 10.0%. Results Compared with the TCE group, the hepatocyte edema in the fat emulsion intervention group was significantly reduced. The levels of ALT and AST in serum and MDA and SOD in liver homogenate of TCE group were higher than that of control group and fat emulsion intervention group respectively (P <0.05). The above four indexes of fat emulsion intervention group were higher than those of control group Group (P <0.05). There was no significant difference in the level of GSH-Px between the three groups (P> 0.05). Conclusion Lipid emulsion may reduce TCE-induced acute liver injury in rats by improving lipid peroxidation.