烯醇化酶(Erlolase,2-phospho-D-g;uceratehydrolyase,EC 4.2.1.11)在糖酵解过程中催化2-磷酸甘油与磷酸烯醇丙酮酸间的反应。在哺乳类,烯醇化酶以3种不同的亚基(α、β、γ)构成5种有活性的二聚体形式的同工酶(αα、ββ、γγ、αβ、αγ)。而ββ和αβ(β-烯醇化酶)则特异地存在于心肌和骨骼肌内。故β-亚基是诊断心肌和肌肉组织疾病的特异抗原。我们试图建立免疫法来定最测定血清β-烯醇化酶。本文介绍了烯醇化酶中β-亚基的分离纯化,并制备了特异抗β-亚基的抗体,为免疫法的建立提供了基础。 Erlolase (2-phospho-D-g; uceratehydrolyase, EC 4.2.1.11) catalyzes the reaction between 2-phosphoglycerol and phosphoenolpyruvate during glycolysis. In mammals, enolase forms five active dimeric forms of the isoenzyme (αα, ββ, γγ, αβ, αγ) in three different subunits (α, β, γ). While ββ and αβ (β-enolase) are specifically found in myocardium and skeletal muscle. Therefore, β-subunit is a specific antigen for the diagnosis of myocardial and muscular tissue diseases. We tried to establish an immunoassay to determine the serum β-enolase. This article describes the separation and purification of β-subunit in enolase and the preparation of antibodies specific to β-subunit, which provides a basis for the establishment of immunoassay.