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目的探讨脂肪酸去饱和酶1(FADS1)基因多态性、深海鱼油摄入及其交互作用与口腔鳞状细胞癌(OSCC)的关系。方法 2010年9月—2014年9月,采用病例-对照研究设计,收集福建医科大学附属第一医院经病理确诊的口腔鳞状细胞癌新发病例259例和按年龄、性别频数匹配的对照538例,采用自制调查表收集研究对象的一般情况、饮食习惯如深海鱼油摄入情况等资料,利用Taq Man SNP探针检测FADS1 rs174549基因型。应用非条件Logistic回归模型评估FADS1 rs174549基因多态性与深海鱼油摄入对口腔鳞状细胞癌发病风险的影响,并进行基因-环境交互作用分析。结果携带FADS1 rs174549 AA基因型可显著降低口腔鳞状细胞癌的发生风险(共显性模型:OR=0.53,95%CI 0.33~0.85;隐性模型:OR=0.57,95%CI 0.38~0.87)。与未补充深海鱼油者相比,规律地补充深海鱼油可以降低OSCC的发生风险(OR=0.54,95%CI 0.32~0.91)。此外,FADS1 rs174549基因多态性与补充深海鱼油之间存在正相乘交互作用(OR_(相乘)=0.31,95%CI 0.11~0.87)。结论FADS1rs174549 AA基因型和深海鱼油摄入是口腔鳞状细胞癌发生的保护因素,且二者之间存在相乘交互作用。
Objective To investigate the association of fatty acid desaturase 1 (FADS1) gene polymorphism, fish oil intake and their interaction with oral squamous cell carcinoma (OSCC). Methods From September 2010 to September 2014, a case-control study was designed to collect 259 new cases of pathologically diagnosed oral squamous cell carcinoma in the First Affiliated Hospital of Fujian Medical University and 538 matched by age and sex frequency For example, using the self-made questionnaire to collect the general information of the subjects, dietary habits such as the intake of fish oil, etc., the FADS1 rs174549 genotype was detected by Taq Man SNP probe. Non-conditional Logistic regression model was used to evaluate the association between FADS1 rs174549 gene polymorphism and the incidence of oral squamous cell carcinoma in deep-sea fish oil, and to analyze the gene-environment interaction. Results AA genotype of FADS1 rs174549 could significantly reduce the risk of oral squamous cell carcinoma (OR = 0.53, 95% CI 0.33-0.85; recessive model: OR = 0.57, 95% CI 0.38-0.87) . Regular supplementation of deep-sea fish oil reduced the risk of OSCC (OR = 0.54, 95% CI 0.32 to 0.91) compared with those who did not. In addition, there was a positive reciprocal interaction between FADS1 rs174549 polymorphism and deep-sea fish oil (OR_ (multiplication) = 0.31, 95% CI 0.11-0.87). Conclusion The FADS1rs174549 AA genotype and the intake of fish oil are the protective factors of oral squamous cell carcinoma, and there is a multiplicative interaction between the two.