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The unique temporal expression patte of miR-200c in epididymis during post-natal development in juvenile rats was revealed by our home-made miRNA microarray in this paper.It was found that miR-200c expressed in the lowest level at Day 7 and then increased to the highest at Day 36 followed by a dramatic decrease.The patte was exactly inverse to that of mRNA expression of transcrip tion factor 8(TCF8)revealed by quantitative real-time polymerase chain reaction(qRT-PCR),providing an extra evidence that TCF8 is one degradation target of miR-200c even in epididymis.Moreover,the qRT-PCR study on expression of E-cadherin and interleukin-2 indicated that miR-200c does exert an obvious effect on the mRNA expression of E-cadherin by directly regulating the mRNA level of TCF8,although the effect on interleukin-2 is not obvious as on E-cadherin,which implicates that interleukin-2 may be also regulated by other factors besides TCF8 in rat epididymis.