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以正己烷为流动相 ,添加一定比例的异丙醇作为改性剂 ,在纤维素 三 (3,5 二甲基苯基氨基甲酸酯 ) (CDMPC)手性固定相上 ,实现了对己唑醇光学异构体的高效液相色谱直接拆分 ,研究了流动相中异丙醇的比例对分离效果的影响 ,优化了色谱拆分条件 ,进行了机理的初步探讨。
Using n-hexane as the mobile phase, a certain proportion of isopropanol was added as a modifier to achieve a good performance on the cellulose tris (3,5-dimethylphenylcarbamate) (CDMPC) chiral stationary phase The optical resolution of azole alcohol was resolved by HPLC. The influence of the ratio of isopropanol in the mobile phase on the separation efficiency was studied. The chromatographic conditions were optimized and the mechanism was discussed.