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目的:探讨松生拟层孔菌抗肿瘤作用及其机制。方法:采用乙醇提取与硅胶柱层析分离,并对其乙醇粗提物、石油醚相、乙酸乙酯相提取物分别进行了体内(小鼠移植性肿瘤),外(采用Alamar Blue法)抗肿瘤活性研究。结果:松生拟层孔菌的乙醇粗提物,石油醚相,乙醇乙酯相在100ug/ml时,体外对NCI-H460细胞株具有增殖抑制作用,100mg/kg时对在体的H22瘤细胞具有显著的抗肿瘤活性,并能诱导H22瘤细胞凋亡,显著提高荷瘤鼠血清中IL-2、TNF-a的水平。结论:松生拟层孔菌乙醇提取物具有抗肿瘤作用,并能增强机体的免疫功能。
Objective: To explore the anti-tumor effect and mechanism of Pinus pinaster. Methods: Ethanol extraction and silica gel column chromatography were used. The ethanol extract, petroleum ether phase and ethyl acetate extract were used in vivo (mouse transplanted tumor) and external (using Alamar Blue method) Tumor activity studies. Results: The ethanol extracts of Pinus pinaster, petroleum ether and ethyl acetate phases inhibited the proliferation of NCI-H460 cells in vitro at 100ug / ml. The inhibitory effect of 100mg / kg on the growth of H22 tumor cells It has significant anti-tumor activity, induces apoptosis of H22 tumor cells and significantly increases the levels of IL-2 and TNF-a in the serum of tumor-bearing mice. Conclusion: Ethanol extract from Pinus pinaster has anti-tumor effect and can enhance the body’s immune function.